Phylogenetic Positioning within Proteobacteria: From Betaproteobacteria to Alcaligenaceae

Abstract

The genus Ampullimonas comprises one species named Ampullimonas aquatilis. The organism forms rod-shaped cells that are 1.0 µm in width and 1.0–2.0 µm in length. The species is motile. The organism stains Gram-negative and endospores are not observed. Colonies are nonpigmented. The species A. aquatilis is mesophilic and strictly aerobic. Cytochrome c oxidase positive and catalase negative. Chemoorganotrophic; capable of chemolithoheterotrophic growth with molecular hydrogen. Able to fix N2 under microaerobic conditions. Growth factors are necessary for growth in minimal medium. Organic acids are used as carbon and energy sources; sugars, amino acids and polyols tested are not used for growth with the exception of glycerol. Major respiratory quinone of this species is ubiquinone 8 (U-8). The polar lipid profile is composed of phosphatidylglycerol (PG) and phosphatidylethanolamine (PE). Fatty acids are primarily straight-chain saturated and monounsaturated; hydroxy fatty acids are also present. The type strain was isolated from bottled mineral water. DNA G+C content (mol%): 49.7–49.8 (HPLC). Type species: Ampullimonas aquatilis França et al. 2016VP.